ZRB2695

Anti-Ferritin Heavy Chain Antibody, clone 1J22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Anti-CHRM4 Antibody, clone 18C7.2 ZooMAb^® Mouse Monoclonal, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, FTH1, Ferritin H subunit, Ferritin heavy chain
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1J22, recombinant monoclonal
• Application: IF, IHC, WB
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J22, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 21.23 kDa, observed mol wt ~20 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_002023.2
• UniProt accession no.: P02794
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... FTH1(2495)

Beschreibung

General description

Ferritin heavy chain (UniProt: P02794; also known as EC:1.16.3.1, Ferritin H subunit, Cell proliferation-inducing gene 15 protein) is encoded by the FTH1 (also known as FTH, FTHL6, OK/SW-cl.84, PIG15) gene (Gene ID: 2495) in human. Ferritin is an oligomeric (24 oligomers) protein that is widely expressed and is found predominantly in the liver in hepatocytes. It is also detected in spleen, skeletal muscles, and bone marrow. It stores iron in a soluble, non-toxic, readily available form and releases it in a controlled manner. Hence, it acts as a buffer against iron deficiency and iron overload. It prevents free iron concentrations from increasing in the cytosolic and nuclear compartments. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also mediates iron uptake in capsule cells of the developing kidney. It is present in two types of subunits: The light (L) chain and heavy (H) chain. In human liver, H-chain is shown to be the predominant

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Ferritin Heavy Chain (FTH).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ferritin heavy chain in Rat liver tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ferritin Heavy Chain in Human Liver and Mouse Spleen tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Ferritin Heavy Chain in Human liver tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ferritin Heavy Chain in human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1J22 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Ferritin Heavy Chain. It targets an epitope within 16 amino acids from the N-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1