ZRB2671

Anti-CYP46A1 Antibody, clone 1C20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): CH24H, Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase, Cytochrome P450 46A1
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1C20, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 56.82 kDa; observed mol wt ~56 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: mouse, human
• Speziesreaktivität (Voraussage durch Homologie): monkey
• Verpackung: antibody small pack of 25
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Methode(n): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgGκ
• Epitopsequenz: Internal
• Protein-ID-Hinterlegungsnummer: NP_006659.1
• UniProt-Hinterlegungsnummer: Q9Y6A2
• Grünere Alternativprodukt-Kategorie: Aligned
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... CYP46A1(10858)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CYP46A1. It targets an epitope within 21 amino acids from the internal region of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the Internal region of human CYP46A1.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in U-251 MG cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CYP46A1 in U-251 MG cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CYP46A1 in lysate from human brain tissue and mouse brain tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CYP46A1 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CYP46A1 in U-251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound CYP46A1 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Cholesterol 24-hydroxylase (UniProt: Q9Y6A2; EC:1.14.14.25; also known as CH24H, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase, Cytochrome P450 46A1) is encoded by the CYP46A1 (also known as CYP46) gene (Gene ID: 10858) in human. CYP46A1 is a member of the cytochrome P450 superfamily of enzymes. These enzymes are characterized by their heme-binding domain, which is crucial for their enzymatic activity involving electron transfer. CYP46A1 is a membrane-associated protein predominantly located in the endoplasmic reticulum. Its structure facilitates the oxidation of cholesterol, a critical step in cholesterol homeostasis and metabolism. The primary function of CYP46A1 is to catalyze the conversion of cholesterol to 24S-hydroxycholesterol. This conversion is a key mechanism for the elimination of cholesterol from the brain, as 24S-hydroxycholesterol can cross the blood-brain barrier, unlike cholesterol itself. This enzymatic activity is vital for maintaining cholesterol balance within the central nervous system and influences various cellular processes including membrane fluidity and the formation of lipid rafts. Alterations in CYP46A1 activity and expression have been implicated in several neurodegenerative diseases. Reduced activity of CYP46A1 has been associated with Alzheimer s disease, potentially due to impaired cholesterol turnover in the brain, which affects the generation and clearance of amyloid-beta plaques.

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable