ZRB2047

Anti-CBX1 Antibody, clone 1D7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Chromobox protein homolog 1;HP1Hsbeta;Heterochromatin protein 1 homolog beta;HP1 beta;Heterochromatin protein p25;M31;Modifier 1 protein;p25beta
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1D7, recombinant monoclonal
• Application: ICC, IHC, WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D7, recombinant monoclonal
• description: 1D7 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 21.42 kDa, observed mol wt ~22 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): canine, monkey, porcine, feline, bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001120700
• UniProt accession no.: P83916
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CBX1(10951)

Beschreibung

General description

Chromobox protein homolog 1 (UniProt: P83916; also known as HP1Hsbeta, Heterochromatin protein 1 homolog beta, HP1 beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25beta, CBX1) is encoded by the CBX1 (also known as CBX) gene (Gene ID: 10951) in human. CBX1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It recognizes and binds histone H3 tails methylated at Lys-9 via the chromo domain, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX1 preferentially recognizes H3K9me3 (peptide binding specificity Kd = 5 M). However, substitution of threonine 6 to alanine reduces its affinity significantly (Kd = 45 M). CBX1 is reported to possess oncogenic activities in hepatocellular carcinoma (HCC). Its expression is significantly increased in HCC and higher expression has been linked to larger tumor size, poor tumor differentiation, and tumor vascular invasion. It interacts with transcription factor HMGA2 to activate the Wnt/β-Catenin signaling pathway. Suppression of β-Catenin is shown to abolish CBX1-mediated cell growth. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yang, Y., et al. (2018). Transl. Oncol. 11(5); 1110-1118; Kaustov, L., et al. (2011). J. Biol. Chem. 286(1): 521-529).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chromobox protein homolog 1 (CBX1).

Application

Quality Control Testing

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX1 in Caco-2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX1 in HeLa, L6, and NIH3T3 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX1 in A431, HepG2, and NIH3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX1 in human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CBX1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1D7 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Chromobox protein homolog 1 (CBX1). It targets an epitope within 15 amino acids from the C-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable