ZMS1078

Anti-CFTR Antibody, clone MM13-4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Cystic fibrosis transmembrane conductance regulator;ATP-binding cassette sub-family C member 7;Channel conductance-controlling ATPase;cAMP-dependent chloride channel
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: MM13-4, recombinant monoclonal
• Application: FACS, ICC, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MM13-4, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 168.14 kDa, observed mol wt ~170 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): monkey, porcine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NM_000492
• UniProt accession no.: P13569
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CFTR(1080)

Beschreibung

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Cystic fibrosis transmembrane conductance regulator (UniProt: P13569; also known as CFTR, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel) is encoded by the CFTR (also known as ABCC7) gene (Gene ID: 1080) in human. CFTR is a multi-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. CFTR is an epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. It mediates the transport of chloride ions across the cell membrane. This channel is also reported to be permeable to bicarbonate ions. Its channel activity is coupled to ATP hydrolysis. It is shown to play an important role in airway fluid homeostasis and contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby has an important role in defense against pathogens. CFTR channel is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. Mutations in CFTR gene are reported to cause Cystic fibrosis that is characterized by chronic bronchopulmonary disease with recurrent respiratory infections and pancreatic insufficiency. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shah, VS., et al. (2016). Science. 351(6272):503-507; Shcheynikov, N., et al. (2004). J. Biol. Chem. 279(21); 21857-21865).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Immunogen

A linear peptide corresponding to 12 amino acids from the N-terminal, cytoplasmic domain of human Cystic fibrosis transmembrane conductance regulator (CFTR).

Application

Quality Control Testing

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFTR in CFTR transfected human bronchial epithelial cell lysate.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected CFTR in one million CFTR transfected human bronchial epithelial cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CFTR in CFTR transfected bronchial epithelial cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.

Biochem/physiol Actions

Clone MM13-4 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detect CFTR. It targets an epitope within 12 amino acids from the N-terminal, first cytoplasmic domain.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1