Z0276

Anti-phospho-ZAP-70 (pTyr³¹⁵/pTyr³¹⁹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• UNSPSC Code: 12352203
• MDL number: MFCD07370447

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human, mouse
• technique(s): western blot: 1:1,000 using Jurkat cells treated with H₂O₂.
• UniProt accession no.: P43403
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... ZAP70(7535) ; mouse ... Zap70(22637)

Beschreibung

General description

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the ζ chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling. Phosphorylation of Tyr³¹⁵ and Tyr³¹⁹ residues regulates TCR-mediated signal transduction . Anti-phospho-ZAP-70 pTyr³¹⁵/pTyr³¹⁹) antibody is specific for human ZAP-70. The product is expected to react with mouse ZAP-70 (as its 100% homologous to human ZAP-70), but has not been tested for it.

Immunogen

synthetic phosphopeptide derived from the region of human ZAP-70 that contains tyrosines 315 and 319.

Application

Anti-phospho-ZAP-70 pTyr³¹⁵/pTyr³¹⁹) antibody is suitable for use in western blot (1:1,000 using Jurkat cells treated with H₂O₂ and immunoblot.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)