W3269

Monoclonal Anti-WDR62 antibody produced in mouse

~1.0 mg/mL, clone 3G8, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-C19orf14, Anti-WD repeat domain 62
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 3G8, monoclonal
• Application: IF, WB
• Citations: 5

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3G8, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~170 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 0.5-1 μg/mL using HEK-293T cells over expressing human WDR62, western blot: 0.5-1 μg/mL using HEK-293T cells over expressing human WDR62
• isotype: IgG2a
• UniProt accession no.: O43379
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... WDR62(284403)

Beschreibung

General description

Monoclonal Anti-WDR62 (mouse IgG2a isotype) is derived from the hybridoma 3G8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein encoding a fragment of human WDR62. WD repeat 62 is a member of WD-repeats and contains 15 WD repeats. WD-repeats consists of minimally conserved domains of 40-60 amino acids, which are initiated by glycine-histidine (GH) dipeptide 11 to 24 residues from the N-terminus and ends with tryptophan-aspartic acid (WD) dipeptide at the C terminus.

WDR62 contains 15 WD repeats and has been identified in aphosphoproteome analysis as a protein associated with the mitotic spindle. WD40 proteins are implicated in many essential biologicalfunctions including adaptor/regulatory modules in signaltran

Application

Monoclonal Anti-WDR62 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

WD repeat 62 (WDR62) is identified in a phosphoproteome analysis as a protein associated with the mitotic spindle. It physically interacts with RNA-binding protein (RALY), TATA-binding protein (TBP), C1 or f103, mitogen-activated protein kinase (MAPK9) and itself. WDR62 potentiates c-Jun N-terminal kinase (JNK) activity. Mutations in this gene are linked to microcephaly and cortical malformations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable