V9139

Anti-Visfatin (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-NAmPRTase, Anti-NMPT, Anti-Nicotinamide-phosphoribosyltransferase, Anti-PBEF1, Anti-Pre-B-cell colony enhancing factor 1
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~50 kDa
• Speziesreaktivität: rat, human, mouse (predicted)
• Konzentration: ~1.5 mg/mL
• Methode(n): western blot: 1-2 μg/mL using A549 cell lysate; western blot: 2-4 μg/mL using rat lung extract (S1 fraction)
• UniProt-Hinterlegungsnummer: P43490
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NAMPT(10135) ; mouse ... Nampt(59027) ; rat ... Nampt(297508)

Beschreibung

Allgemeine Beschreibung

The gene for visfatin (also known as pre-B colony enhancing factor 1, PBEF1, nicotinamide phosphoribosyl transferase, NAMPT), is mapped to human chromosome 7q22.3. The encoded protein is an adipokine produced from adipocytes. It is a highly conserved protein and is also secreted by lymphocytes. Visfatin is expressed in large amounts in the bone marrow, liver tissue, and muscle and is also present in heart, placenta, lung, and kidney tissues.

Spezifität

Anti-Visfatin (C- terminal) recognizes human and rat visfatin.

Anwendung

Anti-Visfatin (C-terminal) antibody produced in rabbit has been used in:

• western blotting(10)
• immunohistochemistry
• immunofluorescence

Biochem./physiol. Wirkung

Visfatin is an enzyme that catalyzes the biosynthesis of nicotinamide adeninine dinucleotide (NAD) from nicotinamide. A variety of signaling pathways are regulated by visfatin including phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT), signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated protein kinase (ERK1/2). It is also reported to regulate the activity of sirtuin 1 (SIRT1) and protect the mammalian cells from death. Since visfatin catalyses a major rate-limiting step in the production of NAD, it is crucial in metabolism, carcinogenesis and immune responses. Visfatin also can act as cytokine and regulates the expression of vascular endothelial growth factor (VEGF) in the proliferation of endothelial cells and formation of capillary tubes. Deregulation of visfatin has been implicated in diabetes mellitus, obesity, rheumatoid arthritis and chronic hepatitis

Visfatin is involved in the regulation of energy homeostasis. Visfatin has been shown to activate its target cells by binding to the insulin receptor at a site distinct from insulin, and its expression level in plasma increases during the development of obesity. Visfatin can induce the cellular expression of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-7,(11) and IL-6.

Zielbeschreibung

Visfatin (C-terminal) (also known as pre-B colony enhancing factor1, PBEF1, nicotinamide phosphoribosyltransferase, NAMPT), is a highly conserved protein involved in the regulation of cellular metabolism and energy homeostasis.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)