V3638

Vascular Endothelial Growth Factor 164 from rat

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(e): VEGF 164, VEGF-164, VEGF₁₆₄, Vasculotropin
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01636149
• Biological source: rat
• Recombinant: expressed in NSO cells
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 50 kDa by SDS-PAGE, monomer 25 kDa by SDS-PAGE
• Impurities: endotoxin, tested

Eigenschaften

• Produktname: Vascular Endothelial Growth Factor 164 from rat, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
• biological source: rat
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.08 ng/mL ED₅₀
• mol wt: dimer 50 kDa by SDS-PAGE, monomer 25 kDa by SDS-PAGE
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: P16612
• storage temp.: −20°C
• Gene Information: rat ... Vegfa(83785)

Beschreibung

Application

Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca²⁺ transients′ measurement.[1] It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.[2]

Biochem/physiol Actions

Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.

Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.[3]

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in human umbilical vein endothelial cells.

Other Notes

164 amino acid variant of rat VEGF.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)