T6424

Trypsin aus meschlichem Pankreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

• Synonym(e): Serine Protease 1
• CAS-Nummer: 9002-07-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-650-8
• MDL number: MFCD01323069
• EG-Nummer: 3.4.21.4 (BRENDA, IUBMB)
• Biological source: human pancreas

Eigenschaften

• biological source: human pancreas
• Quality Segment: 200
• form: salt-free, lyophilized powder
• packaging: vial of ≥1,000 BAEE units
• UniProt accession no.: P07477, P07478, P35030
• storage temp.: −20°C
• Gene Information: human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)

Beschreibung

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Preparation Note

Minimum 1,000 BAEE units/vial

Analysis Note

Proteingehalt mittels E1%/280.

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves