T6277

Anti-Troponin T antibody, Mouse monoclonal

clone JLT-12, purified from hybridoma cell culture

• Synonym(e): Anti-ANM, Anti-NEM5, Anti-STNT, Anti-TNT, Anti-TNTS
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164752
• Conjugate: unconjugated
• Clone: JLT-12, monoclonal
• Application: IHC, WB
• Citations: 87

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: JLT-12, monoclonal
• contains: 15 mM sodium azide
• species reactivity: chicken, rat, bovine, rabbit
• technique(s): immunohistochemistry: suitable, western blot: 1:200 using rabbit total skeletal muscle extract
• isotype: IgG1
• UniProt accession no.: P13805
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Tnnt1(171409), Tnnt2(24837), Tnnt3(24838)

Beschreibung

General description

Monoclonal Anti-Troponin T (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Troponin T is a vital subunit of troponin. Troponin T is a comma or rod shaped protein with a length of 185-205 Å. Troponin T is positioned in the actin helix groove and is extended along the filament of troponin. Life span of serum troponin T is 120 minutes. N- terminal end of the protein is enriched in negatively charged residues and C- terminal end is enriched with positively charged residues. This uneven distribution of charges in troponin T facilitates its aggregation at physiological salt concentration. In humans, cardiac troponin T exists in four isoforms, out of which three are expressed in the fetus and one isoform is expressed in the adult heart.

Immunogen

Troponin T from rabbit skeletal muscle.

Application

Monoclonal Anti-Troponin T antibody produced in mouse has been used in immunostaining.

Monoclonal Anti-Troponin T antibody produced in mouse has been used in following studies:

• Western blot analysis.
• Immunocytochemical analysis.
• Immunofluorescence.

Biochem/physiol Actions

Troponin T is a subunit of troponin, which facilitates binding of troponin to tropomyosin. It also plays a crucial role in muscle contraction. Cardiac troponin T acts as a potential marker for the detection of acute myocardial infarction (MI). Apart from facilitating binding of troponin components to the actin-tropomyosin filament, it is has an essential role in the regulation of actomyosin ATPase activity. Cardiac troponin T gene mutation leads to hypertrophic cardiomyopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable