If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
Fortfahren mit
T3253
Trizma® Hydrochlorid
reagent grade, ≥99.0% (titration), crystalline
Synonym(e):
TRIS -hydrochlorid, TRIS HCl, Tris-(hydroxymethyl)-aminomethan -hydrochlorid, Tromethan-Hydrochlorid
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| Größe/SKU | Verfügbarkeit | Preis |
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Über diesen Artikel
grade
reagent grade
Quality Segment
assay
≥99.0% (titration)
form
crystalline
storage condition
dry at room temperature
technique(s)
affinity chromatography: suitable
impurities
≤0.5% water (Karl Fischer)
color
white
useful pH range
7.0-9.0
pKa (25 °C)
8.1
mp
150-152 °C
solubility
H2O: soluble, clear, colorless
density
1.39 g/cm3 at 20—25 °C, 1.41 g/cm3 at 20—25 °C
absorption
≤0.05 at 290 nm at 40%
suitability
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
General description
Tris-Puffer spielen eine wichtige Rolle bei Verfahren wie Proteinelektrophorese und Western Blot und werden in Puffern mit niedriger Ionenstärke für den Proteintransfer eingesetzt. Sie sind auch integraler Bestandteil von SDS-PAGE-Gelen, Laufpuffern und Blotting-Puffern. Darüber hinaus werden Tris-Salze, insbesondere in TAE- und TBE-Puffern, häufig in der DNA-Agarose-Elektrophorese eingesetzt. Diese Puffer sind von entscheidender Bedeutung für verschiedene Anwendungen, einschließlich Biochemie, Proteinaufreinigung und Elektrophorese, und zeigen die Vielseitigkeit und Bedeutung von Tris-HCl bei der Aufrechterhaltung eines optimalen pH-Werts für die biochemische und zellbiologische Forschung.
Application
Features and Benefits
- Ideal als biologischer Puffer für die Zellbiologie und biochemische Forschung
- Effektive Pufferung mit pH-Werten von 7 bis 9 mit einem pKa-Wert von 8,1 (25 °C)
- In Tests bestätigte niedrige Schwermetallkontamination zur Sicherstellung der Eignung für verschiedene Anwendungen.
Packaging
- Die aktuelle DRYPOUR®-Verpackungskonfiguration ist ein nestbares 50-Liter-Fass
- 25KG-DP stellt die Standard-Fertigverpackung dar
- Kleinere Mengen können für variable Konfigurationen in dasselbe Fass gefüllt werden
- Verbessert die Betriebseffizienz durch Verkürzung der Materialvorbereitungszeit und Erhöhung der Bedienersicherheit
- Minimiert das Anbacken hygroskopischer Salze
- Bietet dreifachen Schutz: vor Feuchtigkeit innen und außen sowie vor Verunreinigungen
- Dieses hochmoderne Verpackungssystem minimiert das Anbacken und verkürzt so den Zeitaufwand für die Materialaufbereitung erheblich
- PE-Fass mit unserem neuen Sicherheitsverschluss, einer Polyethylen-Innenhülle mit integrierten Trockenmittelbeuteln und einer atmungsaktiven Tyvek®-Innenhülle
- Die Trockenmittelbeutel sind fest in die Polyethylen-Innenhülle integriert
- Der dreifache Schutz bietet zwei unschätzbare Vorteile:
- Deutlich weniger Anbacken
- Keine Kontaminationsgefahr durch das Trockenmittel
Other Notes
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description reagent grade, ≥99.0% (titration), crystalline | description pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration) | description BioPerformance Certified, suitable for cell culture, ≥99.0% (titration) | description EMPROVE® EVOLVE |
| assay ≥99.0% (titration) | assay ≥99.0% (titration) | assay ≥99.0% (titration) | assay - |
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) protein extraction: suitable, cell culture | mammalian: suitable, electrophoresis: suitable, DNA purification: suitable | technique(s) cell culture | mammalian: suitable |
| grade reagent grade | grade - | grade BioPerformance Certified | grade - |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| form crystalline | form powder | form crystalline powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
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Lagerklasse
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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Is this a simple mixture of Tris-base & HCl ? If I want to adjust both the concentration and pH of Tris-HCl, can I achieve the same effect by purchasing two reagents and preparing the solution myself?
1 answer-
This product is trizma® hydrochloride. This product is not a mixture of Trizma® hydrochloride and Trizma® base.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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How can I find the technical bulletin 106B that is referenced in the Product Information Sheet for Product T3253, Trizma® hydrochloride?
1 answer-
Under the Documents section above.
Helpful?
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What is the pH of Trizma HCl, Product T3253, in solution?
1 answer-
The pH of Trizma hydrochloride in water is dependent on the concentration. The pH of a 0.1 M aqueous solution is approximately 4.7 while the pH of a 40% (w/w) aqueous solution is typically 2.6-3.3.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product T3253, Trizma® hydrochloride, the same as Tris buffer?
1 answer-
No. Trizma HCl in solution at 0.05 M will produce a pH of approximately 4.7, but will have little if any buffering capacity. Trizma Base in solution at 0.05 M will produce a pH of approximately 10.4, but will have little if any buffering capacity. Blending Trizma Base and Trizma HCl will produce any desired pH between 7 and 9, with a reasonable buffering capacity.
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