T1763

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

• UNSPSC Code: 12352204
• eCl@ss: 42020142
• NACRES: NA.54
• EG-Nummer: 3.4.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00082094
• Biological source: bovine pancreas (trypsin)
• Concentration: ≥15 units/mL (packed gel)

Eigenschaften

• biological source: bovine pancreas (trypsin)
• Quality Segment: 200
• form: buffered aqueous suspension
• concentration: ≥15 units/mL (packed gel)
• extent of labeling: ≥15 units per mL packed gel
• matrix: cross-linked beaded agarose
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2

Other Notes

Insolubilized

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H317,H319,H335
• pcodes: P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter