SRP6522

Ubiquitin-TAMRA-labeled (TMR-Ub)

≥95% (SDS-PAGE)

• Synonym(e): CEP80, HUBCEP80, RPS27A, Ribosomal Protein S27a, UBA80, UBB, UBCEP1, UBCEP80
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Mol-Gew.: 9.133 kDa
• Verpackung: pkg of 50 μg
• Konzentration: 8 mg/mL
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Physikalische Form

≥ 8 mg/mL in PBS.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable