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SRP6484

Carbonic Anhydrase 2/CA2 human

Name: Carbonic Anhydrase 2/CA2 human
Brand: SIGMA

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(e):

CA-II, CAII, Car2

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NACRES:

NA.32

UNSPSC Code:

12352204

Biological source:

human

Recombinant:

expressed in HEK 293 cells

Assay:

≥95% (SDS-PAGE)

Form:

lyophilized

Mol wt:

calculated mol wt 30 kDa, observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)

Impurities:

<1 EU/μg endotoxin (LAL test)

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Unterstützung erhalten

Eigenschaften

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 30 kDa, observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)

packaging

pkg of 10 μg, pkg of 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P00918

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CA2(760)

Beschreibung

General description

Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.

Application

Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).

Biochem/physiol Actions

Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.

Physical form

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.