SRP6426

E-Cadherin/ECAD/Cadherin-1/CD324 Protein human

recombinant, expressed in HEK 293 cells, ≥90% (SDS-PAGE)

• Synonym(e): Arc-1, CDH1, LCAM, UVO
• NACRES: NA.32
• UNSPSC Code: 12352202
• Biological source: human
• Form: lyophilized
• Mol wt: calculated mol wt 67 kDa, observed mol wt 67 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 23 is the predicted N-terminal.)
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• sterility: sterile
• assay: ≥90% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 67 kDa, observed mol wt 67 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 23 is the predicted N-terminal.)
• packaging: pkg of 25 μg
• UniProt accession no.: P12830
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CDH1(999)

Beschreibung

General description

Cadherin-1 (CDH1) is a transmembrane protein, which is mainly involved in cell adhesion in normal epithelia. The associated gene is mapped to human chromosome 16q22.1.

Biochem/physiol Actions

Cadherin-1 (CDH1) stabilizes the cell adhesion in normal cells and also helps in signaling via association with β catenin. Mutations in the CDH1 gene are linked with oral clefts in the Thai population. CDH1 works as a tumor suppressor in various cancers. Low expression of CDH1 increases cellular invasive and metastatic potential. For instance, low expression of this gene is associated with poor survival of patients with esophageal cancer.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable