SRP5232

EIF2S1, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): EIF-2, EIF-2α, EIF-2A, EIF2, EIF2A
• CAS-Nummer: 480740-00-9
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: ~40 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~40 kDa
• NCBI accession no.: NM_004094
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EIF2S1(1965)

Beschreibung

General description

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) is a 35kDa protein. It is the α subunit of the eukaryotic initiation factor 2 (eIF2α). The gene encoding it is localized on human chromosome 14q23.3.

Biochem/physiol Actions

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. The Ser51 in the mature human EIF2-α protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-α also plays an essential role in erythropoiesis.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H360D
• pcodes: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable