SRP4702

CTGF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(e): connective tissue growth factor
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD07370810

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 501.0-2.0 μg/mL ED₅₀
• mol wt: ~11.2 kDa
• packaging: pkg of 20 μg
• impurities: endotoxin, tested
• NCBI accession no.: NM_001901
• UniProt accession no.: P29279
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CTGF(1490)

Beschreibung

General description

The gene CTGF (connective tissue growth factor) is mapped to human chromosome 6q23.2. CTGF is a member of the CCN (Cyr61, CTGF , Nov) family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. The protein has an N-terminal secretory signal peptide and four domains (an insulin-like growth factor-binding protein domain, a Von Willebrand factor domain, a thrombospondin type-1 repeat module, and a CT). It is expressed in various tissues, such as connective tissue, heart, brain, kidney, lung, liver, muscle, pancreas gland and placenta. Recombinant human CTGF is a 11.2Da protein of 98 amino acid residues.

Biochem/physiol Actions

CTGF (connective tissue growth factor) stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells.[1] It binds to IGF (insulin growth factor), TGF (transforming growth factor) β1 and BMP (bone morphogenetic protein)-4. CTGF plays a crucial role in the progression of diabetic nephropathy. It can work as an oncoprotein in glioma and melanoma. However, it works as a tumor suppressor in lung cancer and colon cancer.

Physical form

Sterile filtered and Lyophilized without additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable