SRP4011

Cardiotrophin-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): CT1, CTF1, Cardiophin 1
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD01865988

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~22.5 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: Q16619
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CTF1(1489)

Beschreibung

General description

Cardiotrophin-1 belongs to the interleukin-6 (IL-6) family of cytokines. It is a cytoprotective protein. The gene is mapped to human chromosome 16p11.
Recombinant human Cardiotrophin-1 is a 22.5kDa protein containing 200 amino acid residues.

Biochem/physiol Actions

Cardiotrophin-1 signals via β receptor glycoprotein 130 (gp130) and β receptor leukemia inhibitory factor receptor (LIFR). It confers protective role on cardiac myocytes. Cardiotrophin-1 protects cardiomyocytes from cell death, however, it can also induce cardiac hypertrophy. It is also involved in neural tissue development and helps in protection of the mature nervous system from injuries and dysfunction. Cardiotrophin-1 also protects liver against injury. It is also involved in the control of body weight and intermediate metabolism. In mouse model, it protects β cells from apoptosis. It also promotes glucose-mediated insulin secretion. Absence of the cardiotrophin-1 gene in mice is associated with obesity, hyperglycemia, hyperinsulinemia and hypercholesterolemia.

Physical form

Sterile filtered and then lyophilized in 1 x PBS.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in 20 mM Tris, pH 8.0 to a concentration of <1.0 mg/mL. The solution can be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable