SRP3330

IL-6 from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): BSF-2, HPGF, IFN-?2, B cell differentiation factor (BCDF)
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 5x10^7 units/mg ED₅₀
• mol wt: 21.7 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• color: white to off-white
• UniProt accession no.: P08505
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... IL6(16193)

Beschreibung

General description

Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 188 amino acid residues.

Application

IL-6 from mouse has been used to study IL-6-mediated inhibition of mouse preadipocyte differentiation.

Biochem/physiol Actions

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R and gp130.

Physical form

Lyophilized from 0.1% TFA

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable