SRP3129

NT-4 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(e): Neurotrophic 4/5 (NT-4/NT-5), Neurotrophin-4
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 20-50 ng/mL ED₅₀
• mol wt: 14.0 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P34130
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NTF4(4909)

Beschreibung

General description

NT-4 is a neurotrophic factor structurally related to b-NGF, BDNF, and NT-3. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-4 is expressed in the prostate, thymus, placenta and skeletal muscle. NT-4 can signal through the LNGFR and trkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant human NT-4 is a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers (260 total amino acid residues).

Biochem/physiol Actions

NT-4 is a neurotrophic factor structurally related to b-NGF, BDNF, and NT-3. Recombinant human NT-4 is a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers (260 total amino acid residues).

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable