SRP2118

RNA Polymerase II, RPB10 subunit, GST tagged human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Synonym(e): RBP10, RPABC5, RPB10, RPB10beta, RPB7.6, hRPB7.6, hsRPB10b
• NACRES: NA.26
• UNSPSC Code: 12352202
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥85% (SDS-PAGE)
• Form: frozen liquid
• Mol wt: ~33.6 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥85% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~33.6 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 300 μg/mL
• color: clear colorless
• NCBI accession no.: NM_021128
• UniProt accession no.: P62875
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... POLR2L(5441)

Beschreibung

Biochem/physiol Actions

The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. It is a zinc-binding protein with an atypical CX2CXnCC metal binding motif. RPB10 participates in protein-protein interactions with additional components of the polymerase holoenzyme. Experimentally, the binding of RPB10 to an RPB3-RPB11 (or RPAC40-RPAC19 in RNAPI/III) heterodimer is well characterized and persists in both eukaryal and archaeal RNAPs. Binding of RPB10 to this a2-like heterodimer through zinc-mediated hemi-coordination suggests an early role in holoenzyme assembly since the formation of the α2-complex is the first step in the assembly of the prokaryotic RNAPs.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable