SRP2009

TFIIF (RAP30+Rap74) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(e): BTF4
• NACRES: NA.26
• UNSPSC Code: 12352202
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• Form: frozen liquid
• Mol wt: ~84 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~84 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 400 μg/mL
• color: clear colorless
• NCBI accession no.: NM_002096, NM_004128
• UniProt accession no.: P13984,P35269
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2F1(2962), GTF2F2(2963)

Beschreibung

Biochem/physiol Actions

The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP34) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The Rap74 subunit of TFIIF can be phosphorylated by TAF250 both in vivo and in vitro. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E. coli s factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable