SRP0722

KRAS (Mature Form), Active

PRECISIO^® Kinase, recombinant

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: frozen liquid
• Assay: 70-95% (SDS-PAGE)
• Recombinant: expressed in E. coli
• Mol wt: ~23 kDa

Eigenschaften

• recombinant: expressed in E. coli
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 2.0-3.5 nmol/min·mg (GTPase-Glo)
• mol wt: ~23 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_033360
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KRAS(3845)

Beschreibung

Application

Useful for enzyme assay and Western blot.

Biochem/physiol Actions

Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking ⁽¹⁾. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types ⁽²⁾. Other members of the RAS superfamily may also contribute to cancer.

Preparation Note

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• Gefahrencodes: H315,H319,H360D
• Sicherheitshinweise: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable