SRP0388

EZH2 /EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: aqueous solution
• Assay: ≥90% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: 48 kDa (AEBP2), 51 kDa (EED), 53 kDa (AEBP2), 86 kDa (EZH2), 87 kDa (SUZ12)

Eigenschaften

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 48 kDa (AEBP2), 51 kDa (EED), 53 kDa (AEBP2), 86 kDa (EZH2), 87 kDa (SUZ12)
• packaging: pkg of 20 μg
• NCBI accession no.: NM_003797 (EED), NM_004456 (EZH2), NM_005610 (RbAP48), NM_015355 (SUZ12), NM_153207 (AEBP2)
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EED(8726), EZH2(2146), RBBP4(5928), SUZ12(23512)

Beschreibung

General description

Inactive mutant version of our regular EZH2 5-member complex, but with a Tyr-to-Cys mutation on amino acids 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end ; Y646C*) with N-terminal His-tag, MW= 86 kDa, human EED (GenBank Accession No. NM_003797) (amino acids 2-end) with N-terminal Flag-tag, MW= 51 kDa, human SUZ12 (GenBank Accession No. NM_015355) (amino acids 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (GenBank Accession No. NM_153207) (amino acids 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (GenBank Accession No. NM_005610) (amino acids 2-end) with N-terminal His-tag, MW = 48 kDa, coexpressed in Baculovirus expression system. *This mutation is often cited as Y641C in variant 3 (GenBank Accession No. NM_001203247).

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H360D
• pcodes: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklasse: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable