SAB4200328

Anti-acetyl-Histone H3 [Ac-Lys⁵⁶]

IgG fraction of antiserum

• Synonym(e): Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3K56ac, Anti-H3t, Anti-HIST3H3
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 10

Eigenschaften

• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~15 kDa
• species reactivity: rat, human, mouse
• technique(s): western blot: 1:1000-1:2000 using NIH3T3 or HeLa cell lysates
• UniProt accession no.: Q16695
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: acetylation (Lys56)
• Gene Information: human ... HIST3H3(8290)

Beschreibung

General description

Histones are considered as the important constituents of chromatin. They are classified into core histones and linker histones. Histone H3 gene codes for a H3 protein sequence. This H3 gene is located on human chromosome 1 (1q42).

Immunogen

synthetic peptide containing acetylated Lys⁵⁶ of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3.

Application

Anti-acetyl-Histone H3 [Ac-Lys56] has been used in western blot analysis.

Biochem/physiol Actions

Core histones and linker histones are known possesses inter and intra-specific amino acid sequence variability. Histone modifications are thought to play an important role in cancer and disease. Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. In human embryonic stem cells, histone H3 Lys56 acetylation is associated to the core transcriptional network.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable