S5897

Sephadex^® G-50

BioReagent, DNA grade, for molecular biology, fine

• CAS-Nummer: 9004-54-0
• MDL-Nummer: MFCD00132771
• UNSPSC-Code: 23151817
• NACRES: NA.52

Eigenschaften

• Qualität: Molecular Biology; for molecular biology
• Produktlinie: BioReagent
• Form: beads
• Methode(n): DNA purification: suitable
• Matrix: cross-linked dextran
• Quellen: 1 g swells to 9-11 mL
• Perlendurchmesser: 20-80 μm
• Porengröße: exclusion limit (20 bp dsDNA (rA)₂₀)
• pH-Wert: 2-10
• Fremdaktivität: DNase, RNase, none detected
• Lagertemp.: room temp
• SMILES String: O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
• InChI: 1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
• InChIKey: FZWBNHMXJMCXLU-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Sephadex^® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Anwendung

Sephadex^® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.[1]

Leistungsmerkmale und Vorteile

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)