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Merck

S5816

Sigma-Aldrich

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(e):

SDF-1α/PBSF

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.77

Biologische Quelle

mouse

Qualitätsniveau

Rekombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

Form

powder

Wirksamkeit

0.15-0.6 ng/mL ED50

Qualität

endotoxin tested

Mol-Gew.

predicted mol wt ~8 kDa

Verpackung

pkg of 10 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

<1 EU/μgtested (LAL test)

UniProt-Hinterlegungsnummer

Lagertemp.

−20°C

Angaben zum Gen

mouse ... Cxcl12(20315)

Allgemeine Beschreibung

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

Anwendung

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

Biochem./physiol. Wirkung

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..
Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Angaben zur Herstellung

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Hinweis zur Analyse

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Shin J, et al.
Diabetes, 67(6), 1068-1078 (2018)
CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
Krohn A, et al.
Cancer Letters, 280, 65-71 (2009)
Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
Huang YC, et al.
Acta Biomaterialia, 8, 1048-1056 (2012)
The Cytokine CXCL12 Promotes Basket Interneuron Inhibitory Synapses in the Medial Prefrontal Cortex.
Wu PR, et al.
Cerebral Cortex, 27(9), 4303-4313 (2017)
Yoshitsugu Takabatake et al.
Journal of the American Society of Nephrology : JASN, 20(8), 1714-1723 (2009-05-16)
CXC chemokine ligand 12 (CXCL12; stromal cell-derived factor 1) is a unique homeostatic chemokine that signals through its cognate receptor, CXCR4. CXCL12/CXCR4 signaling is essential for the formation of blood vessels in the gastrointestinal tract during development, but its contribution

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