S2822

Anti-Synapsin II in Kaninchen hergestellte Antikörper

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-SYN2, Anti-SYNAPSIN 2, Anti-SYNII, Anti-SYNIIa, Anti-SYNIIb
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 5

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~54 kDa (synapsin IIb), antigen ~74 kDa (synapsin IIa)
• species reactivity: mouse, human, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using extracts of HEK-293T cells expressing recombinant human synapsin II, mouse brain extract (S1 fraction), and rat PC12 cell lysate
• UniProt accession no.: Q92777
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SYN2(6854) ; mouse ... Syn2(20965) ; rat ... Syn2(29179)

Beschreibung

General description

Synapsin II (SYN-2) is a neuronal phosphoprotein present in synaptic vesicles. The SYN-2 gene is mapped to human chromosome 3p25.2. Structurally, synapsin types have a conserved N -terminal region and vary in their C-terminal region.

Immunogen

synthetic peptide corresponding to amino acids 438-453 located near the C-terminus of rat synapsin II, conjugated to KLH. This sequence is identical in rat synapsin IIa and IIb isoforms and mouse synapsin II, and is highly conserved in human synapsin IIa and IIb (one amino acid substitution).

Application

Anti-Synapsin II antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Anti-Synapsin II specifically recognizes human, rat, and mouse synapsin IIa and Iib.

Synapsins regulate synaptic vesicle clustering, neurotransmitter release, and the formation of synaptic terminals. Synapsin II is involved in the formation of synaptic terminals during neuronal development. Similar to synapsin I, synapsin II can link synaptic vesicles to the actin cytoskeleton, thus regulating the availability of synaptic vesicles for exocytosis. Synapsin II association with synaptic vesicles is controlled by phosphorylation. Synapsin II is proposed as a candidate associated with increased vulnerability to schizophrenia. It is also implicated in synaptogenesis and neurotransmitter release impairment in multiple neuropsychiatric diseases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)