RAB0431

Mouse Shh-N ELISA Kit

for serum, plasma and cell culture supernatant

• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Shh(20423)
• Input: sample type cell culture supernatant(s); sample type serum; sample type plasma
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type serum; sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 pg/mL; standard curve range: 4.1-1000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Shh(20423)

Beschreibung

General description

The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse Sonic Hedgehog (N-terminus)

Application

For research use only. Not for use in diagnostic procedures.
Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level.

Biochem/physiol Actions

Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDB
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids