RAB0275

Mouse IL-1 β ELISA Kit

for cell and tissue lysates

• Synonym(e): Cytokine detection kit
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Il1b(16176)
• Input: sample type cell lysate; sample type tissue lysate
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 pg/mL; standard curve range: 2.74-2000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il1b(16176)

Beschreibung

General description

The Mouse IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 β cell lysate and tissue lysate.

Immunogen

Recombinant Mouse IL-1 β

Application

Mouse IL-1 β ELISA Kit has been used to measure the concentration of interleukin 1 (IL)1-β in serum and bronchoalveolar lavage fluid (BALF) sample.

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Interleukin 1 β (IL-1β) stimulates the production of endogenous 92 kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). It is a proinflammatory cytokine that induces various acute and chronic inflammation. Therefore, IL-1β can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β brings neutrophil and macrophage to the site of infection. Over expression of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. Mutations in IL-1β gene leads to renal manifestations and renal sequelae in Henoch-Schonlein purpura (HSP).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDB
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDB
• RABLYSIS1

  2X Cell Lysis Buffer (Item J)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids