RAB0245

Mouse IL-10 ELISA Kit

for serum, plasma and cell culture supernatant

• Synonym(e): IL-10, Interleukin-10
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Il10(16153)
• Input: sample type cell culture supernatant(s); sample type serum; sample type plasma
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type serum; sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 45 pg/mL; standard curve range: 78.13-5000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il10(16153)

Beschreibung

General description

The Mouse IL-10 (Interleukin-10) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IL-10

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine. In mouse, IL-10 produced by the CD4⁺ Th2 (T helper cell 2) subset, inhibits the expression of cytokines specifically IFN-3 (interferon), by Thl (T helper cell 1) clones. mIL-10 has pleiotropic property and it interacts with various cell types such as thymocytes, cytotoxic T cells, mast cells, B cells, and macrophages. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. B- cell specific IL-10 might have a suppressive role in systemic lupus erythematosus (SLE).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDB
• RABELADD

  ELISA 5X Assay/Sample Diluent Buffer D (Item K)

  SDB
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDB
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDB
• RABWASH4

  20X Wash Buffer (Item B)

  SDB

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids