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RAB0210

Mouse Granzyme B ELISA Kit

for serum, plasma and cell culture supernatant

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PackungsgrößeSKUVerfügbarkeitPreis
1 kit
Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein)
€ 637,00

Über diesen Artikel

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Gzmb(14939)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice

€ 637,00


Versand in der Regel in 1 Woche. (Bei Bestellungen außerhalb der USA und Europas rechnen Sie bitte zusätzlich 1-2 Wochen für die Lieferung ein)

Technischer Dienst
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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Gzmb(14939)

General description

The Mouse Granzyme B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Granzyme B in serum, plasma, cell culture supernatants and urine.
The Mouse Granzyme B enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of mouse granzyme B in serum, plasma, cell culture supernatants and urine. Granzyme B binds to mannose-6-phosphate/insulin-like growth factor II receptor. This protein is implicated in fast DNA fragmentation, a key step towards apoptosis in cells. Granzyme B is involved in the cleaving of pro-caspase-3, which leads to the activation of caspase-3. This protein also cleaves caspase-6, -7, -8, -9, and -10.[1]

Immunogen

Recombinant Mouse Granzyme B

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Dieser Artikel
RAB1498RAB0560RAB0104
Gene Information

mouse ... Gzmb(14939)

Gene Information

human ... GZMB(3002)

Gene Information

mouse ... Lgals1(16852)

Gene Information

mouse ... Csf3(12985)

species reactivity

mouse

species reactivity

human

species reactivity

mouse

species reactivity

mouse

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.87 pg/mL
standard curve range: 2.87-700 pg/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 12 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.62-150 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable


Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB & Preisangaben

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDB

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDB

  • ELISA Stop Solution (Item I)
    SDB

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDB

  • 20X Wash Buffer (Item B)
    SDB

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Lagerklasse

10 - Combustible liquids



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Analysenzertifikate (COA)

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Verwandter Inhalt


Kosei Hasegawa et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 41(4), 201-207 (2018-02-13)
Cancer immunotherapy has now been established as a leading standard therapeutic option in a subset of patients with cancer. In this study, we conducted a phase I dose-escalation trial using a mixture of 5 peptides to vaccinate cervical cancer patients
Sarah J Lord et al.
Immunological reviews, 193, 31-38 (2003-05-20)
A main pathway used by cytotoxic T lymphocytes (CTLs) and natural killer cells to eliminate pathogenic cells is via exocytosis of granule components in the direction of the target cell, delivering a lethal hit of cytolytic molecules. Amongst these, granzyme
Ciputra Adijaya Hartana et al.
PloS one, 13(7), e0200079-e0200079 (2018-07-03)
The immune system plays a significant role in urothelial bladder cancer (UBC) progression, with CD8+ T cells being capable to directly kill tumor cells using perforin and granzymes. However, tumors avoid immune recognition by escape mechanisms. In this study, we



Global Trade Item Number

SKUGTIN
RAB0210-1KT04061834960645

Questions

  1. It says here that this ELISA kit works for the detection of GzmB in mouse serum, but I can't find any papers that have used serum. Could you please cite a paper or previous work demonstrating the utility of this kit for detecting GzmB in serum?

    1 answer
    1. Unfortunately, published citations on the use of this kit with mouse serum are not available. Please see the image below for an example of expected recovery rates:

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