R8654

Anti-RAD17 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-S.cerevisiae homolog of RAD24, Anti-hRad17
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD08705511
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 5

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 75 kDa
• species reactivity: mouse, rat, human
• packaging: antibody small pack of 25 μL
• technique(s): immunoprecipitation (IP): suitable, western blot: 1:500-1:1,000 using HeLa cell lysates
• UniProt accession no.: O75943
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAD17(5884) ; mouse ... Rad17(19356) ; rat ... Rad17(310034)

Beschreibung

General description

Cell cycle checkpoint protein RAD17 is mapped to human chromosome 5q13.2 . It is also called s a replication factor C (RFC)-like DNA damage sensor protein. Eight alternative spliced transcript variants of the gene, which encode four distinct proteins, have been reported.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human RAD17 (isoform 1), conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide differs from that of isoform 2 by two amino acids.

Application

Anti-RAD17 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

RAD17 has been reported in breast, non-small cell lung carcinoma and breast cancer. RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. Treatment of human cells with genotoxic agents induced ATM/ATR dependent phosphorylation of RAD17 at Ser635 and Ser645 , suggesting that phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells.

Physical form

Supplied as a solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)