R8257

Mlu I from Micrococcus luteus (lysodeikticus)

buffered aqueous glycerol solution

• CAS-Nummer: 81458-04-0
• UNSPSC Code: 12352204
• MDL number: MFCD00166590
• EG-Nummer: 3.1.21.4 (BRENDA, IUBMB)

Eigenschaften

• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Disclaimer

Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam⁺ strains).