R7009

Sty I from Escherichia coli strain carrying pST27

buffered aqueous glycerol solution

• CAS-Nummer: 96880-98-7
• UNSPSC Code: 12352204
• NACRES: NA.53
• EG-Nummer: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00166674
• Concentration: 10,000 units/mL

Eigenschaften

• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1% gelatine (v/v), 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).