PLA0310

Rabbit anti-Phospho RPA32 (S33) Antibody, Affinity Purified

• Synonym(e): 32kDa, REPA2, RF-A protein 2, RP-A p32, RP-A p34, RPA32, replication factor A protein 2, replication protein A 34 kDa subunit, replication protein A2, replication protein A2 (32kD)
• NACRES: NA.41
• UNSPSC Code: 12352203
• Species reactivity: human
• Technique(s): western blot: 1:1,000-1:5,000
• Application: WB
• Citations: 5

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• species reactivity: human
• technique(s): western blot: 1:1,000-1:5,000
• accession no.: NP_002937.1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: phosphorylation (pSer33)
• Gene Information: human ... RPA2(6118)

Beschreibung

General description

Replication protein A2 (RPA2) is a 32kDa protein and one of the subunits of replication protein A complex. The gene encoding it is localized on human chromosome 1p35.

Immunogen

Immunogen was a phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33 using the numbering given in entry NP_002937.1 (GeneID 6118).

Biochem/physiol Actions

Replication protein A2 (RPA2) is involved in DNA repair and replication. The protein has been shown to be phosphorylated as a response to DNA damage and apoptosis in vitro.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable