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P4458

Penicillin-Streptomycin

liquid, non-animal origin, suitable for cell culture, BioReagent

Penicillin-Streptomycin
1 of 1 reviewers received a sample product or took part in a promotion

Synonym(e):

Pen-Strep

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Ihnen/SKUVerfügbarkeitPreis
20 mL
Warenkorb auf Verfügbarkeit prüfen
€ 15,70
100 mL
Warenkorb auf Verfügbarkeit prüfen
€ 27,50

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.76

€ 15,70


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Produktname

Penicillin-Streptomycin, Solution Stabilized, with 5,000 units penicillin and 5mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Quality Segment

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

concentration

50 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

mode of action

cell wall synthesis | interferes, protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

SMILES string

CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N

InChI

1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1

InChI key

KMOOCZWLFBSQCW-XFOLRDBHSA-N

General description

Penicillin, a β-lactam antibiotic is extremely active towards bacteria. Penicillins are also termed as penams. It is naturally occurring and has a bicyclic skeleton. Streptomycin is a pseudotrisaccharide. It comprises of a monosubstituted aminocyclitol.

Application

Recommended for use in cell culture media at 20 ml/L.
Penicillin-Streptomycin has been used:
  • as a supplement in Dulbecco′s modified Eagle′s medium (DMEM) to maintain the Huh7 liver hepatoma cell line
  • as a supplement in Roswell park memorial institute-1640 (RPMI) medium to maintain mouse lymphoma YAC-1 cells
  • as a component in minimum essential medium Eagle to treat individual mouse spleens for the preparation of splenocytes
  • as a supplement in FMX-8 complete medium to culture Chinese hamster ovary cells[1]

Biochem/physiol Actions

Penicillins can prevent the synthesis of bacterial cell wall. This antibiotic can be used in the treatment of skin and urinary tract infections, upper and lower respiratory infections, and endocarditis. Streptomycin can block Mycobacterium tuberculosis in vitro. Streptomycin inhibits prokaryote protein synthesis[1] by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Other Notes

20ml,100ml
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.
This product is formulated to contain 5,000 units penicillin and 5 mg streptomycin/mL solubilized in a proprietary citrate buffer.

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Dieser Artikel
11074440001P4333P0781
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

mode of action

protein synthesis | interferes, cell wall synthesis | interferes

mode of action

cell wall synthesis | interferes, protein synthesis | interferes

mode of action

cell wall synthesis | interferes, protein synthesis | interferes

mode of action

cell wall synthesis | interferes, protein synthesis | interferes

product line

BioReagent

product line

-

product line

BioReagent

product line

BioReagent

Quality Level

300

Quality Level

-

Quality Level

300

Quality Level

300

form

liquid

form

lyophilized (clear, colorless solution after reconstitution)

form

liquid

form

liquid


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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–4 of 4 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. What is the concentration of the P4458 Penicillin-Streptomycin solution? For example, is it a 50X or 100X?

    1 answer
    1. Product No. P4458 is designed to be added directly to media; you should add 20mL to 1L of medium.  There is no intermediate dilution step. So the concentration 50X refers to the 50X dilution into media to the final working concentration.

      Helpful?

  3. What is the method of preparation for Product No. P4458 (Penicillin-Streptomycin); what solution is it made in?

    1 answer
    1. Product No. P4458 is not simply penicillin and streptomycin dissolved in water; it is made in a proprietary buffer.

      Helpful?

  4. What is the storage temperature and the stability of the P4458 Penicillin-Streptomycin?

    1 answer
    1. Product No. P4458 (Penicillin-Streptomycin solution) should be stored in the freezer at -20 °C for long-term storage. It may be kept refrigerated at 2-8 °C for up to 40 days.

      Helpful?

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