Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced and is used to study the effects of ionizing radiation on the formation and stability of G-quadruplex structures. This indicates that this product can form G-quadruplexes.
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P4404
Polyganylsäure Kaliumsalz
≥95% (TLC), enzyme from (synthesis), lyophilized powder
Synonym(e):
Poly(G) potassium salt
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Über diesen Artikel
Assay:
≥95% (TLC)
Biological source:
enzyme from (synthesis)
Form:
lyophilized powder
Solubility:
water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow
Storage temp.:
−20°C
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Unterstützung erhaltenProduktname
Polyganylsäure Kaliumsalz, lyophilized powder
biological source
enzyme from (synthesis)
Quality Segment
assay
≥95% (TLC)
form
lyophilized powder
quality
≤5% free nucleotides
solubility
water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
−20°C
General description
Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced in Thermus thermophilus from guanosine diphosphate (GDP) and its synthesis is catalyzed by the enzyme polynucleotide phosphorylase.[1]
Application
Polyguanylic acid (PolyG) is used to study the effects of ionizing radiation on formation and stability of G-quadruplex structures and as a target molecule for physicochemical studies of electronic excitation (vibrational spectra) of guanosine.
Polyguanylic acid potassium salt has been used:
- as a ligand for surface neuropilin-1 (NRP1) for internalization studies[2]
- for intercalation studies with trisubstituted and disubstituted triazole-linked phenyl derivatives CL41, CL42 and CL2r50 using voltammetry method[3]
- as a synthetic polynucleotide for agarose gel electrophoresis analysis[4]
Biochem/physiol Actions
Polyguanylic acid acts as a ligand for scavenger receptor and reduces the surface expression neuropilin-1 in endothelial cells.[2]
Preparation Note
Prepared from GDP using polynucleotide phosphorylase
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Dieser Artikel | |||
|---|---|---|---|
| biological source enzyme from (synthesis) | biological source - | biological source synthetic (organic) | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| assay ≥95% (TLC) | assay ≥98% (TLC) | assay ≥98% (TLC) | assay ≥98% (enzymatic) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| solubility water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow | solubility water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless | solubility water: 4.80-5.20 mg/mL, clear to hazy, colorless to faintly yellow | solubility water: 100 mg/mL, clear, colorless to faintly yellow |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can this form G quadruplex?
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What is the average length of the poly(g) chains?
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Unfortunately, this product is not tested to determine the molecular weight and information is limited. The length of poly(g) chains is not determined. Polyguanylic acid is a mixture of different and wide ranging molecules, therefore the number of bases is not determined. There is no size limiting or selecting step during the production process. Historical information is available on past lots, which were assayed by both LALLS assay and a formaldehyde denaturing system. Analysis using the denaturing system indicates a wide range of <80,000 to 500,000 with the greatest density at <80,000 to 210,000 . Two past lots have been analyzed by LALLS resulting in estimated molecular weights of 385,000 and 482,000 daltons.
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