Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest.
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.
T Takahashi et al.
Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
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P4039
Phospholipase C Peptide
lyophilized powder, Type XIV, ≥150 units/mg protein
Synonym(e):
Lecithinase C, Lipophosphodiesterase I, PC-PLC
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
41106300
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
Produktname
Phospholipase C aus Clostridium perfringens (C. welchii), Type XIV, lyophilized powder, ≥150 units/mg protein
biological source
bacterial (Clostridium perfringens)
Quality Segment
type
Type XIV
form
lyophilized powder
specific activity
≥150 units/mg protein
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
greener alternative category
storage temp.
−20°C
Gene Information
Clostridium perfringens str. 13 ... plc(988262)
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Physical form
Lyophilisiertes Pulver in gepufferten Salzen.
Preparation Note
Chromatographisch aufgereinigt
Other Notes
1 U setzt 1.0 μmol wasserlösliches, organisches Phosphor aus Eigelb L-α-Phosphatidylcholin pro Minute frei bei pH 7.3 und 37°C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| biological source bacterial (Clostridium perfringens) | biological source bacterial (Clostridium perfringens) | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) | Gene Information - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| type Type XIV | type Type I | type - | type - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is it possible for you to disclose the outline of the purification method for P7633 and P4039 (just the name of the method is fine)?
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Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
1 answer-
Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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