N9663

NEK2, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): HsPK21, NLK1
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: buffered aqueous glycerol solution
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~76 kDa

Eigenschaften

• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 237-321 nmol/min·mg
• mol wt: ~76 kDa
• UniProt accession no.: P51955
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... NEK2(4751)

Beschreibung

Biochem/physiol Actions

NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2.NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)