N6539

Anti-NUMB (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-S171
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 8

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen ~70 kDa
• species reactivity: human, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using rat brain extract (S1 fraction) and A431 cell lysate
• UniProt accession no.: P49757
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NUMB(8650) ; mouse ... Numb(18222) ; rat ... Numb(29419)

Beschreibung

General description

Numb gene with 13 exons is mapped to human chromosome 14q24.3. The protein comprises 651 amino acids. It belongs to the clathrin-associated sorting protein family with two domains namely phosphotyrosine-binding (PTB) and proline-rich region (PRR) domain. Mammalian Numb gene gives rise to at least four alternatively spliced transcripts that produce four protein isoforms Numb1-4, ranging from 65 to 72 kDa.

Numb homolog-NUMB is an adaptor protein and is aberrantly expressed in cancers.

Application

Anti-NUMB (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Numb is a critical Notch antagonist and a conserved adapter protein that plays a role in the self-renewal of NSCs and neural differentiation in the central nervous system (CNS). In neural progenitors of CNS, the functional loss of numb is implicated in embryonic lethality accompanied by precocious neuronal differentiation. It has been suggested that a switch in numb isoform expression is a critical step in cortical development. Numb1 or numb3 isoforms that contain an insertion in the proline-rich region (PRRL), promote the proliferation of neural crest stem cells (NCSCs). Numb2 or numb4 isoforms without PRR insertions (PRRS) show ectopic expression and promote neuronal differentiation.

Numb homolog-NUMB may act as a tumor suppressor. It also takes part in determining the cell fate. NUMB is involved in the endocytosis and trafficking of receptors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)