N3665

Pyrimidine Nucleoside Phosphorylase from Bacillus subtilis

recombinant, expressed in E. coli, ≥70 units/mg protein

• Synonym(e): PynP
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 2.4.2.2 (BRENDA, IUBMB)
• Specific activity: ≥70 units/mg protein
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥70 units/mg protein
• storage temp.: −20°C

Beschreibung

General description

The roles of the residues in the catalytic active site of pyrimidine nucleoside phosphorylase from Bacillis subtilis have been elucidated using hybrid quantum-mechanical/molecular-mechanical methods.

Biochem/physiol Actions

Pyrimidine nucleoside phosphorylase functions in the nucleotide synthesis salvage pathway by catalyzing the reversible phosphorolysis of pyrimidines. Both uridine and thymidine are substrates.

Other Notes

One unit will convert 1 μmole each of 2′-deoxyuridine and phosphate to uracil and 2-deoxyribose 1-phosphate per minute at pH 7.4 and 37 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable