N1408

Nerve Growth Factor β Human

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonym(e): HSAN5, NGF-β human, NGFB, hNGFβ
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164982
• Biological source: human
• Recombinant: expressed in NSO cells
• Assay: >97% (SDS-PAGE)
• Form: lyophilized powder
• Impurities: <1 EU/μg

Eigenschaften

• Produktname: NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.15-3.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• packaging: pkg of 5X0.1 mg, pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• solubility: water: soluble 0.1 g/L at 20 °C
• UniProt accession no.: P01138
• storage temp.: −20°C
• Gene Information: human ... NGF(4803)

Beschreibung

General description

Recombinant nerve growth factor β (NGFB) is supplied as a lyophilized protein for use in mammalian cell culture research involving neuronal development and differentiation studies in vitro.

Application

NGF-β human has been used:

• in protein pin arrays
• to induce U87-MG (glioblastoma cell line) cell proliferation
• in drug release kinetics

Biochem/physiol Actions

Nerve Growth Factor‑β (NGF‑β) is a neurotrophic factor that mediates cellular responses related to growth, differentiation, and survival of neuronal and non-neuronal cells. NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Physical form

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Analysis Note

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)