The Trace Elements Ready Made Solution (MBD0056) is a 100x liquid supplement designed to provide vital minerals for bacterial growth in minimal media. It is based on Wolin and Wolfe’s recipe and is suitable for supplementing growth media to support a variety of bacteria. The product is intended to be added to pre-sterilized and cooled growth media at a 1:100 dilution. However, the documentation does not specifically mention its use in M9 minimal medium for 15N-labeled protein expression, nor does it address compatibility with isotope labeling protocols.
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Über diesen Artikel
NACRES:
NA.54
UNSPSC Code:
41106212
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Segment
sterility
0.2 μm filtered
form
liquid
shelf life
2 yr
packaging
pkg of 50 mL
storage condition
protect from light
concentration
100 ×
suitability
suitable for (Media supplement)
storage temp.
2-8°C
General description
Minerals and vitamins are broadly used to supplement growth media in order to support growth of a variety of different bacteria. The growth media mimics the natural environment of the bacteria thus providing an optimal setting for high throughput cultures. Trace elements ready-made solution, MBD0056, is comprised of vital minerals to support critical bacterial development. The solution is based on Wolin and Wolfe’s recipe, which is especially suitable for culturing anaerobic bacteria of the human microbiome ( )
Features and Benefits
- 0.2uM filtered for removal of contaminants
- Saves time in preparation of media
- Improved accuracy: composition and concentrations are analyzed and established by inductively coupled plasma (ICP)
- Ready-made liquid mixture containing all ingredients.
- Decreases exposure to chemical and toxic risks associated with the powder form of the trace elements
Other Notes
50 mL Trace elements solution:
- EDTA : 570 mg/L
- CuSO4. 5H2O :10 mg/L
- AlK(SO4)2 · 12H2O : 20 mg/L
- H3BO3 :10 mg/L
- Na2MoO4. 2H2O : 10 mg/L
- Na2SeO3 : 1 mg/L
- Na2WO4 . 2H2O : 10 mg/L
- NiCl2 . 6H2O : 20 mg/L
- MgSO4 . 7H2O : 3000 mg/L
- MnSO4 . H2O : 500 mg/L
- NaCl : 1000 mg/L
- NH4Fe(SO4)2 · 12H2O :170 mg/L
- Co(NO3)2 . 6H2O : 100 mg/L
- CaCl2 · 2H2O :100 mg/L
- ZnSO4 . 7H2O :100 mg/L
Add to pre-sterilized and cooled growth media. Concentration 100x.
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
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Could you confirm that this product, MBD0056, can be used for M9 minimal medium for 15N-labeled protein expression?
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