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Merck

MBD0014

Sigma-Aldrich

Microbial DNA standard from Pseudomonas Aeruginosa

Suitable for PCR, sequencing and NGS, ≥10 ng/μL

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About This Item

UNSPSC-Code:
41105500
NACRES:
NA.24

Qualitätsniveau

Form

liquid

Konzentration

≥10 ng/μL

Methode(n)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Eignung

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

Versandbedingung

ambient

Lagertemp.

−20°C

Allgemeine Beschreibung

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Pseudomonas Aeruginosa genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Pseudomonas aeruginosa is a gram negative aerobic to facultative anaerobe rod shaped bacteria which commonly inhabits soil and aqueous environments. In cases of a compromised immune system Pseudomonas aeruginosa can be an opportunistic pathogen. Its devastating effect is mostly known in Cystic Fibrosis (CF) patients. in addition, it often poses a major problem in burn wounds, chronic wounds, and in chronic obstructive pulmonary Disorder (COPD). P.aeruginosa forms biofilms on implanted biomaterials within hospital surface and water supplies. The international nosocomial infection control consortium reported that P. aeruginosa nosocomial infections have become a worldwide healthcare issue.




Read here how to use our standards to ensure data integrity for your microbiome research.

Anwendung

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Leistungsmerkmale und Vorteile

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physikalische Form

Liquid -The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Montserrat Saladié et al.
Frontiers in microbiology, 11, 572504-572504 (2020-10-31)
In recent years the study of the commensal microbiota is driving a remarkable paradigm shift in our understanding of human physiology. However, intrinsic technical difficulties associated with investigating the Microbiomics of some body niches are hampering the development of new

Artikel

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

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