MAK545

Beta-Lactamase Activity Assay Kit

sufficient for 200 colorimetric tests

• Synonym(e): β-Lactamase Assay Kit
• NACRES: NA.84
• UNSPSC Code: 41116104

Eigenschaften

• detection method: colorimetric
• relevant disease(s): immunological diseases
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. A β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, deactivating the antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance.

The Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.

Biochem/physiol Actions

The β-lactamase activity is detected using nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase.



One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 µmole of nitrocefin per minute at pH 7.0 at 25°C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H319,H334
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3