M9015

Malachitgrün Oxalat

Dye content, ≥90%, certified by the Biological Stain Commission, powder or crystals

• Synonym(e): N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbeniumoxalat, Echtgrün
• Empirische Formel (Hill-System): C₂₃H₂₅N₂ · C₂HO₄ · 0.5C₂H₂O₄
• CAS-Nummer: 2437-29-8
• Molekulargewicht: 463.50
• NACRES: NA.47
• PubChem Substance ID: 24897308
• UNSPSC Code: 12171500
• EC Number: 219-441-7
• Beilstein/REAXYS Number: 3644550
• Colour Index Number: 42000

Eigenschaften

• Produktname: Malachitgrün Oxalat, certified by the BSC
• agency: certified by the BSC
• Quality Level: 200
• form: powder or crystals
• composition: Dye content, ≥90%
• technique(s): microbe id | staining: suitable
• color: faint green to very dark green
• mp: 144-150 °C
• solubility: H₂O: 1 mg/mL, blue to very deep blue
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
• InChI: 1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
• InChI key: REZFCYOVFCJTDG-UHFFFAOYSA-M

Beschreibung

General description

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.[1]

Application

Zertifiziert für den Einsatz als Gegenfärbung für in Paraffin eingebettetes botanisches Material, als Sporenfarbstoff für Bakterien nach der Methode von Schaeffer und Fulton.

Malachite Green oxalate salt has been used for phosphate quantification.[2]

Malachite Green oxalate salt has been used:

• in the preparation of Alexander staining solution for cell viability test
• in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
• as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method[3]

Other Notes

The molecular mass of malachite green oxalate is expressed as either:
927.00 g/mol - the mass of 2 mol malachite green + 2 mol monohydrogen oxalate + 1 mol dihydrogen oxalate, or
463.50 g/mol - the mass of 1 mol malachite green + 1 mol monohydrogen oxalate + 0.5 mol dihydrogen oxalate

Sicherheitshinweise

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301,H318,H361fd,H410
• pcodes: P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable