This product is the hydrate form of methotrexate. The maximum allowable water content is 12% and is reported on the lot-specific Certificate of Analysis. Dessicated storage is not necessary but is up to the end user's preference to do so. Any resulting moisture loss should be considered when preparing stock solutions. Please see the link below to review the product datasheet for A6770. This product is not tested for cell culture use, but the information contained in the document would apply to M8407.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/853/a6770pis.pdf
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M8407
L-Amethopterin Hydrat
powder, suitable for cell culture, BioReagent
Synonym(e):
4-Amino-10-methylfolsäure Hydrat, L-4-Amino-N10-methylpteroylglutaminsäure Hydrat, Antifolan Hydrat, MTX Hydrat, Methotrexat Hydrat, Methylaminopterin Hydrat
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mg | Warenkorb auf Verfügbarkeit prüfen | € 373,00 |
500 mg | Warenkorb auf Verfügbarkeit prüfen | € 1.530,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C20H22N8O5 · xH2O
CAS-Nummer:
Molekulargewicht:
454.44 (anhydrous basis)
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Technischer Dienst
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Unterstützung erhaltenProduktname
L-Amethopterin Hydrat, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)
product line
BioReagent
Quality Segment
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: insoluble
antibiotic activity spectrum
neoplastics, parasites
mode of action
enzyme | inhibits
storage temp.
−20°C
SMILES string
[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
InChI
1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key
FPJYMUQSRFJSEW-ZOWNYOTGSA-N
Gene Information
human ... DHFR(1719)
Application
Anwendungsbereiche für Methotrexathydrat:
- als Dihydrofolat (DHFR)-Inhibitor in Sphärenbildungs-Assay-Kulturen
- als Komponente von Dulbecco′s Modified Eagle′s Medium (DMEM) für die Auswahl von transfizierten Zellen
- in Assays zur Messung der Viabilität und Co-Kultivierung
Potenter Inhibitor von Dihydrofolatreduktase und Mittel für Antitumor-Studien. Verwendung zur Hemmung der Dihydrofolatreduktase in DHFR-basierten Proteinexpressionssystemen.
Potenter Inhibitor von Dihydrofolatreduktase und Mittel für Antitumor-Studien. Verwendung zur Hemmung der Dihydrofolatreduktase in DHFR-basierten Proteinexpressionssystemen. Auch bei der Behandlung von pyrimethaminresistenten Plasmodium vivax-Malariaparasiten wirksam.
Potenter Inhibitor von Dihydrofolatreduktase und Mittel für Antitumor-Studien. Verwendung zur Hemmung der Dihydrofolatreduktase in DHFR-basierten Proteinexpressionssystemen. Besitzt auch immunsuppressive Wirkungen, z. B. bei rheumatoider Arthritis.
Biochem/physiol Actions
Methotrexat ist ein Folsäure-Antagonist.
Methotrexat wird zur Hemmung der Dihydrofolatreduktase verwendet und für die DHFR-Überexpression ausgewählt.
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| mode of action enzyme | inhibits | mode of action - | mode of action - | mode of action - |
| antibiotic activity spectrum neoplastics, parasites | antibiotic activity spectrum - | antibiotic activity spectrum - | antibiotic activity spectrum - |
| product line BioReagent | product line - | product line - | product line - |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 300 |
| form powder | form powder | form powder | form - |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Does the powder form of this product M8407 (methotrexate hydrate) need to be stored in a desiccator?
1 answer-
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Regarding to the answer for making a solution of Methotrexate hydrate, can we use Sodium Bicarbonate to dissolve Methotrexate hydrate for cell culture?
1 answer-
This product is not directly soluble in water and is tested for solubility in 1M Ammonium hydroxide at 50 mg/mL. This material is also soluble in 1M Sodium hydroxide. Alternatively, anhydrous DMSO may also be used to dissolve this material at 90 mg/ml. An identical Methotrexate hydrate product, A6770, has a datasheet posted to the website. This datasheet included additional information regarding general use and solution stability. Please see the link below to review this document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/853/a6770pis.pdfHelpful?
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How often does Methotrexate hydrate, Product M8407, need to be added to the media during selection?
1 answer-
Fresh methotrexate should be added each time the culture media is changed. Regardless of cell growth, most media will need to be changed at least once a week.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I make a solution of Methotrexate hydrate, Product M8407?
1 answer-
Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.
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How do I sterilize a solution of Methotrexate hydrate, Product M8407?
1 answer-
A solution of methotrexate can be sterile filtered using a 0.22 mm filter
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How do I store a stock solution of Methotrexate hydrate, Product M8407?
1 answer-
A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.
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