M1821

Anti-Monoamin-Oxidase B in Kaninchen hergestellte Antikörper

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Adrenalin oxidase, Anti-MAO, brain, Anti-MAO, platelet, Anti-Tyramine oxidase
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 9

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~58 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.5 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using rat liver mitochondrial fraction
• UniProt accession no.: P27338
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ASCC3(10973), MAOB(4129) ; rat ... Maob(25750)

Beschreibung

General description

Monoamine Oxidase B (MAO-B) is a mitochondrial flavo enzyme. It is mainly found in the brain in dopaminergic, serotonergic, and histaminergic neurons and astrocytes.

Monoamine Oxidase B is encoded by the gene mapped to human chromosome Xp11.3.

Application

Anti-Monoamine Oxidase B antibody has been used in western blotting.[1]

Anti-Monoamine Oxidase B antibody produced in rabbit has also been used in coimmunoprecipitation (co-IP) and immunohistochemistry.[2]

Biochem/physiol Actions

Anti-Monoamine Oxidase B specifically recognizes human, mouse, and rat MAO-B.

Monoamine oxidase B (MAO-B) is considered the predominant isoform responsible for dopamine metabolism in the human central nervous system (CNS). MAO-B activity correlates with personality traits. Alterations in MAO-B activity may underlie dopamine pathobiology in major depression. Selective MAO-B inhibitors elevate synaptosomal dopamine concentrations, and have recently shown clinical efficacy in the treatment of early Parkinson′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)