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Hanahan′s Broth (SOB Medium)

Liquid microbial growth medium

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UNSPSC Code:
12352200


grade

Molecular Biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

General description

Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Hanahan′s (SOB) media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Physical form

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

Other Notes

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4


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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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